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GAS Reporter (Luc)-HeLa Cell Line (IFNγ/JAK/STAT1 Pathway)
GAS Reporter (Luc)-HeLa Cell Line (IFNγ/JAK/STAT1 Pathway)
Artikelnummer
BPS79041
Verpackungseinheit
2 vials
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Applications: Monitor interferon gamma-induced signal transduction pathways. Screen for activators or inhibitors of JAK/STAT1 signaling pathway using the GAS Reporter Cell Line.
Description: The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of interferon gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the interferon gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind interferon gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.
Host Cell Line: HeLa
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.
Storage Stability: Immediately upon receipt, store in liquid nitrogen.
Supplied As: Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Decker T, Kovarik P, Meinke A. (1997) GAS elements: a few nucleotides with a majorimpact on cytokine-induced gene expression. J Interferon Cytokine Res. 17(3): 121-34.2. Darnell J, Kerr IM, Stark GR. (1994) Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science 264: 1415-1421.
Applications: Monitor interferon gamma-induced signal transduction pathways. Screen for activators or inhibitors of JAK/STAT1 signaling pathway using the GAS Reporter Cell Line.
Description: The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of interferon gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the interferon gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind interferon gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.
Host Cell Line: HeLa
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.
Storage Stability: Immediately upon receipt, store in liquid nitrogen.
Supplied As: Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Decker T, Kovarik P, Meinke A. (1997) GAS elements: a few nucleotides with a majorimpact on cytokine-induced gene expression. J Interferon Cytokine Res. 17(3): 121-34.2. Darnell J, Kerr IM, Stark GR. (1994) Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science 264: 1415-1421.
| Artikelnummer | BPS79041 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 79041 |
| Verpackungseinheit | 2 vials |
| Mengeneinheit | PAK |
| Wirt | Human |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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