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Goat anti Mouse IgG2a (subclass specific), conjugated with Biotin
Goat anti Mouse IgG2a (subclass specific), conjugated with Biotin, Polyclonal, Clone: Polyclonal
Artikelnummer
GAM/IgG2a/Bio
Verpackungseinheit
1 ml
Hersteller
Nordic-MUbio
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Clone: Polyclonal
Background: The reactivity of the antiserum is directed to the subclass IgG2a. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG2a at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2a antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG2a isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2a in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be usedThis immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Pools of purified homogenous IgG2a isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgG2a, subclass specific
Formulation: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Background: The reactivity of the antiserum is directed to the subclass IgG2a. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG2a at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2a antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG2a isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2a in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be usedThis immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Pools of purified homogenous IgG2a isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgG2a, subclass specific
Formulation: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
| Artikelnummer | GAM/IgG2a/Bio |
|---|---|
| Hersteller | Nordic-MUbio |
| Hersteller Artikelnummer | GAM/IgG2a/Bio |
| Green Labware | Nein |
| Verpackungseinheit | 1 ml |
| Mengeneinheit | STK |
| Reaktivität | Mouse (Murine) |
| Klonalität | Polyclonal |
| Methode | Immunohistochemistry (paraffin), Western Blotting, ELISA, Immunocytochemistry, Dot Blot |
| Wirt | Goat |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
