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Goat anti Rat IgG1 (subclass specific), conjugated with Horseradish peroxidase
Goat anti Rat IgG1 (subclass specific), conjugated with Horseradish peroxidase, Polyclonal, Clone: Polyclonal
Artikelnummer
GARa/IgG1/PO
Verpackungseinheit
1 ml
Hersteller
Nordic-MUbio
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Clone: Polyclonal
Background: The reactivity of the antiserum is directed to the subclass IgG1. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG1 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG1 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG1 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG1 in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Pools of purified homogenous IgG1 isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG1, subclass specific.
Formulation: Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Background: The reactivity of the antiserum is directed to the subclass IgG1. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG1 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG1 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG1 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG1 in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Pools of purified homogenous IgG1 isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG1, subclass specific.
Formulation: Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
| Artikelnummer | GARa/IgG1/PO |
|---|---|
| Hersteller | Nordic-MUbio |
| Hersteller Artikelnummer | GARa/IgG1/PO |
| Green Labware | Nein |
| Verpackungseinheit | 1 ml |
| Mengeneinheit | STK |
| Reaktivität | Rat (Rattus) |
| Klonalität | Polyclonal |
| Methode | Immunohistochemistry (paraffin), Western Blotting, ELISA, Immunocytochemistry, Dot Blot |
| Wirt | Goat |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
