HCV1a (R155K), FLAG-His-tags Recombinant

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Encompassing Amino Acids: 21-32 of NS4A, linker (4 a.a.) and a.a. 3-181 of NS3

Amino Acid Sequence: MDYKDDDDKHHHHHHGCVVIVGRIVLSGSGSITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCINGVCWTVYHGAGTRTIASPKGPVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGHAVGLFRAAVCTRGVAKAVDFIPVENLETTMRS

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Description: Fusion protein corresponding to theserine protease NS3 (a.a. 3-181) andcofactor NS4A (a.a. 21-32) from HepatitisC virus genotype 1a (HCV1a), GenBankAccession No. NC_004102, with Arg-to-Lys mutation on a.a. 155, and N-terminalFLAG-His tag, MW = 22.4 kDa,expressed in an E. coli expressionsystem.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20,20% glycerol, and 300 mM imidazole

Genbank: NC_004102

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-His-tags

Uniprot: P27958

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Lin, C., et al., Hepatitis C Virus. Norfolk (UK): Horizon Bioscience; 2006. Chapter 6.
2. Prabhu, R., et al., Exp Mol Pathol. 2004 Jun;76(3):242-52.

• Artikelnummer: BPS80101
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80101
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK