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HCV1b (D168V), FLAG-His-tags Recombinant
HCV1b (D168V), FLAG-His-tags Recombinant
Artikelnummer
BPS80110
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 21-32 of NS4A, linker (3 a.a.), a.a. 31-181 of NS3
Assay Conditions: Assay was performed in 100 µl HCV assay buffer containing 50 mM Tris, pH 7.4, 150 mM NaCl, 5 mM DTT, 10% glycerol, and 5 µM fluorescently labeled HCV substrate peptide. Reaction was continuously monitored at room temperature for 20 min. at ex350/em500.
Description: Hepatitis C virus (HCV) genotype 1b, also known as HCV1b, is a fusion protein of a.a. 21-32 of NS4A, linker (4 a.a.) and a.a. 3-181 of NS3 (GenBank Accession No. AF054247) with Asp-to-Val mutation on a.a. 168, and N-terminal FLAG-His-tag, MW = 22.3 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 400 mM NaCl, 0.2% Triton X-100, 10 mM beta-mercaptoethanol, 100 µg/ml FLAG peptide, and 30% glycerol
Genbank: AF054247
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-His-tags
Uniprot: O92972
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Failla, C., et al., J. Virol. 1994 June;68(6):3753-3760.
2. Lang Kuhs, K.A., et al., Mol. Ther. 2012 May;20(3):669-678.
3. Massariol, MJ., et al., Biochem Biophys Res Commun. 2010 Jan 1;391(1):692-697.
Encompassing Amino Acids: 21-32 of NS4A, linker (3 a.a.), a.a. 31-181 of NS3
Assay Conditions: Assay was performed in 100 µl HCV assay buffer containing 50 mM Tris, pH 7.4, 150 mM NaCl, 5 mM DTT, 10% glycerol, and 5 µM fluorescently labeled HCV substrate peptide. Reaction was continuously monitored at room temperature for 20 min. at ex350/em500.
Description: Hepatitis C virus (HCV) genotype 1b, also known as HCV1b, is a fusion protein of a.a. 21-32 of NS4A, linker (4 a.a.) and a.a. 3-181 of NS3 (GenBank Accession No. AF054247) with Asp-to-Val mutation on a.a. 168, and N-terminal FLAG-His-tag, MW = 22.3 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 400 mM NaCl, 0.2% Triton X-100, 10 mM beta-mercaptoethanol, 100 µg/ml FLAG peptide, and 30% glycerol
Genbank: AF054247
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-His-tags
Uniprot: O92972
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Failla, C., et al., J. Virol. 1994 June;68(6):3753-3760.
2. Lang Kuhs, K.A., et al., Mol. Ther. 2012 May;20(3):669-678.
3. Massariol, MJ., et al., Biochem Biophys Res Commun. 2010 Jan 1;391(1):692-697.
| Artikelnummer | BPS80110 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 80110 |
| Green Labware | Nein |
| Verpackungseinheit | 20 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
