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HDAC10, FLAG-tag Recombinant
HDAC10, FLAG-tag Recombinant
Artikelnummer
BPS50060
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-631
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 25 mM Tris/Cl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and 40 ng/µl HDAC10. Incubation condition: 30 min. at 37°C.
Description: Human HDAC10 (a.a. 2-631) (GenBank Accession No. NM_032019) with N-terminal FLAG-tag, MW= 67 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124mM NaCl, 2.4 mM KCl, 0.05% Tween-20 and10% glycerol.
Genbank: NM_032019
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: Q969S8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chakraborty S. (2006) J. Biol. Chem. 281(46):35070-80.
2. Wolfgang, F. (2001) J. Biol. Chem. 276(38): 35826-35835.
Application Reference(s):
1. Suppression of monosodium urate crystal-induced cytokine production by butyrate is mediated by the inhibition of class I histone deacetylases (2015)
2. The effect of various zinc binding groups on inhibition of histone deacetylases 1-11 (2014)
Encompassing Amino Acids: 2-631
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 25 mM Tris/Cl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and 40 ng/µl HDAC10. Incubation condition: 30 min. at 37°C.
Description: Human HDAC10 (a.a. 2-631) (GenBank Accession No. NM_032019) with N-terminal FLAG-tag, MW= 67 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124mM NaCl, 2.4 mM KCl, 0.05% Tween-20 and10% glycerol.
Genbank: NM_032019
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: Q969S8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chakraborty S. (2006) J. Biol. Chem. 281(46):35070-80.
2. Wolfgang, F. (2001) J. Biol. Chem. 276(38): 35826-35835.
Application Reference(s):
1. Suppression of monosodium urate crystal-induced cytokine production by butyrate is mediated by the inhibition of class I histone deacetylases (2015)
2. The effect of various zinc binding groups on inhibition of histone deacetylases 1-11 (2014)
| Artikelnummer | BPS50060 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 50060 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
