HDAC3/NcoR2, His-Tag, GST-Tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: full length HDAC3, 395- 489 NCOR2

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and HDAC3/NcoR2. Incubation condition: 30 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

Description: Complex of human HDAC3 , GenBank Accession No. NM_003883, full length with C-terminal His-tag, MW= 49.7 kDa, and human NCOR2 , a.a. 395- 489, GenBank Accession No. NM_006312, N-terminal GST-tag, MW= 37.6 kDa, co-expressed in baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 200 mM imidazole

Genbank: NM_003883

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: O15379

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Gregoire, S. et al. Mol. Cell. Biol. 27 (4), 1280-1295 (2007)
2. Escaffit, F. et al., Mol. Cell. Biol. 27 (2), 554-567 (2007)Application References:
1. Wang, Y., et al. Chem Biol. 2015 Feb 19;22(2):273-84. doi: 10.1016/j.chembiol.2014.12.015. Identification of histone deacetylase inhibitors with benzoylhydrazide scaffold that selectively inhibit class I histone deacetylases (2015)
2. Mechanism of Action of 2-Aminobenzamide HDAC Inhibitors in Reversing Gene Silencing in Friedreich's Ataxia (2015)
3. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)
4. Discovery of a small molecule agonist of phosphatidylinositol 3-kinase p110α that reactivates latent HIV-1 (2014)
5. A chimeric SERM-histone deacetylase inhibitor approach to breast cancer therapy (2014)
6.Wang, C., et al. J Med Chem. 2014 Oct 9;57(19):7999-8009. doi: 10.1021/jm500872p. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
7. Selective HDAC Inhibition for the Disruption of Latent HIV-1 Infection (2014)
8. Class I HDAC imaging using [ (3)H]CI-994 autoradiography (2013)
9. Fass, D.M., et al. Neuropharmacology. 2013 Jan; 64: 81–96. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013)
10. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)
11. Baud, MGJ, et al. ChemMedChem. 2013 Jan;8(1):149-56. doi: 10.1002/cmdc.201200450. Highly ligand efficient and selective N-2-(Thioethyl)picolinamide histone deacetylase inhibitors inspired by the natural product psammaplin A (2013)
12. Imaging epigenetic regulation by histone deacetylases in the brain using PET/MRI with ¹¸F-FAHA (2013)
13. Marek, M., et al. PLoS Pathog. 2013;9(9):e1003645. doi: 10.1371/journal.ppat.1003645. Structural basis for the inhibition of histone deacetylase 8 (HDAC8), a key epigenetic player in the blood fluke Schistosoma mansoni (2013)

• Artikelnummer: BPS50003
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50003
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK
• Wirt: Insect