HDAC4, GST-tag, His-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 627-1084 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC4. Incubation condition: 30 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

Description: Human HDAC4 (GenBank Accession No. NM_006037), a.a. 627-1084 (end) with N-terminal GST-tag and C-terminal His-tag, MW= 76 kDa, expressed in baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione,10% glycerol.

Genbank: NM_006037

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag, C-terminal His-tag

Uniprot: P56524

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Leong, H. et al. (2005) Mol. Endocrinol. 19, 2930-2942.2. Zhao et al., (2005) Mol. Cell. Biol. 25, 8456-8464.3. Wang, C., et al. J Med Chem. 2014 Oct 9;57(19):7999-8009. doi: 10.1021/jm500872p. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)

• Artikelnummer: BPS50004
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50004
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect