HDAC5, GST-Tag, His-Tag Recombinant

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Encompassing Amino Acids: 1-1122

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 1 (Catalog #50040), and HDAC5 (0.3125 - 20 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.

Description: Human HDAC5 (GenBank Accession No. NM_005474), a.a. 1-1122 (end) with N-terminal GST-tag and C-terminal His-tag, expressed in a HEK293 expression system. MW=149 kDa.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16mM Glutathione, 20% glycerol.

Genbank: NM_005474

Purity: ≥55%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag, C-terminal His-tag

Uniprot: Q9UQL6

Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Spiegelberg, B.D. and Hamm, H.E. (2005) J. Biol. Chem. 280, 41769-417762. Waltregny et al., (2004) J. Neural Transm. 48, 273-290.

• Artikelnummer: BPS100102
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100102
• Green Labware: Nein
• Verpackungseinheit: 5 µg
• Mengeneinheit: STK