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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 25 mM Tris/HCl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mMMgCl2, and 0.1 mg/ml BSA, 20 µMBPS HDAC substrate 3 (Catalog#50037), and HDAC6 (3.1 - 200 ng).Incubation condition: 30 min at 37°Cfollowed by developing for 15 min atroom temperature.
Description: Human HDAC6, GenBank Accession No. BC069243, full length with N-terminal GST-tag, MW= 159 kDa, expressed in baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 50 mM KCl, 2 mM EDTA, 10% glycerol, and 2 mM DTT
Genbank: BC069243
Host Cell Line: Sf9 cells
Purification: Protein was purified by affinity chromatography and gel filtration.