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HER2, GST-Tag Recombinant
HER2, GST-Tag Recombinant
Artikelnummer
BPS40230
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 679-1255
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in Kinase buffer containing 1 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Human Her2 (GenBank Accession No. X03363), a.a. 679-1255(end) with N-terminal GST-tag, MW=89.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT, and 20% glycerol.
Genbank: X03363
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P04626
Unit Definition: One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Meyer, M. et al., EMBO J. 18, 363-374 (1999).
2. Rahimi, N. et al., J. Biol Chem 275, 16986- 16992 (2000).
Encompassing Amino Acids: 679-1255
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in Kinase buffer containing 1 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Human Her2 (GenBank Accession No. X03363), a.a. 679-1255(end) with N-terminal GST-tag, MW=89.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT, and 20% glycerol.
Genbank: X03363
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P04626
Unit Definition: One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Meyer, M. et al., EMBO J. 18, 363-374 (1999).
2. Rahimi, N. et al., J. Biol Chem 275, 16986- 16992 (2000).
| Artikelnummer | BPS40230 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40230 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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