Histone H3-H4 Tetramer H3k27cMe3, His-tag Recombinant

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Applications: A perfect substrate for histone methyltransferases and demethylases.

Description: Human recombinant histone tetramerconsisting of 2 molecules each ofhistones H3 (a.a. 2-136(end)) and H4(a.a. 2-103(end)), expressed in an E. coliexpression system. Histone H3 hasK27C, C97A, and C111A mutations andwas alkylated to produce the lysineanalog, trimethyl aminoethylcysteine.GenBank Accession Nos. NM_003532,and NM_003548, respectively. Eachhistone protein has an N-terminal Histag.Global MW = 57 kDa.

Format: Aqueous buffer solution

Formulation: 45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol

Genbank: NM_033445, NM_003528, NM_003532, and NM_003548

Host Cell Line: E. coli

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P68431

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Simon, M.D., et al. Cell 2007; 128: 1003-1012.
2. Raut, V.V., Pandey, S.M., Sainis, J.K. Ann Bot. 2011; 108(7):1235-46

• Artikelnummer: BPS52087
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 52087
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK