Histone H4 Antibody

Application Note: Anti-Histone H4 pan Antibody is tested for Western Blot and Dot Blots. This antibody is suitable for Chromatin Immunoprecipitation and ELISA. Expect a single band approximately 11.3 kDa in size corresponding to H4 by western blot in the appropriate tissue or cell lysate. Specific conditions for reactivity should be optimized by the end user.

Concentration Value: 90 mg/mL

ELISA Dilution: 1:20000

Western Blot Dilution: 1:500-1:2000

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: Anti-Histone H4 pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminal region of histone H4.

Other Performance Data: Dot Blot: 1:1000

Physical State: Liquid (sterile filtered)

Purity and Specificity: Anti-Histone H4 pan Antibody antiserum is directed against Histone H4. Cross reactivity with other species was not tested.

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC100-401-BR9
• Hersteller: Rockland
• Hersteller Artikelnummer: 100-401-BR9
• Verpackungseinheit: 100 µL
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Methode: Western Blotting, Dot Blot
• Isotyp: Antiserum
• Human Gene ID: 121504
• Wirt: Rabbit
• Konjugat: Unconjugated