Histone Octamer H3K27cMe3, His-tag Recombinant

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Applications: A perfect substrate for histone methyltransferases and demethylases.

Background: The histone octamer is composed of a central heterotetramer of two copies of both histones H3 and H4, flanked by two heterodimers of histones H2A and H2B. The octamer assembles when the H3/H4 tetramer complexes with two H2A/H2B dimers. These histones of the histone octamer all contain N-terminal tails that emanate from their central histone folds and are targets for epigenetic modification.

Description: Human recombinant histone octamer consisting of 2 molecules each of histones H2A (a.a. 1-130(end)), H2B (a.a. 1-126(end)), H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), expressed in an E. coli expression system. Each histone protein has an N-terminal His-tag. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, trimethyl aminoethylcysteine. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Global MW = 116 kDa.

Format: Aqueous buffer solution

Formulation: 45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol

Genbank: NM_033445, NM_003528, NM_003532, and NM_003548

Host Cell Line: E. coli

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P68431

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Simon, M.D., et al. Cell 2007; 128: 1003-1012.
2. Raut, V.V., Pandey, S.M., Sainis, J.K. Ann Bot. 2011; 108(7):1235-46

• Artikelnummer: BPS52091
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 52091
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK