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Hrd1, SUMO-His-tags Recombinant
Hrd1, SUMO-His-tags Recombinant
Artikelnummer
BPS81051
Verpackungseinheit
25 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 236-617
Applications: Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
Background: Hrd1 is a RING domain E3 ligase that is involved in the conjugation of ubiquitin to target substrates. Hrd1 has been demonstrated to function with the E2 enzymes UBE2D3 (UbcH5c) and UBE2G2 in vitro. Hrd1 is an ER-associated ligase involved in ERAD and has been linked to rheumatoid arthritis. It is present in human rheumatoid synovial cells and found to be a causative factor for arthropathy in in vivo studies. Hrd1 is also found in brain neurons and its proper regulation may be linked to neurodegenerative diseases. This construct is a Hrd1 N-terminal deletion containing residues 246-617 consisting of the complete C-terminal cytoplasmic portion. The N-terminal transmembrane spanning portion has been deleted. This Hrd1 N-terminal deletion mutant is active as an E3 ligase containing the RING and substrate recognition domains.
Biological Activity: Typical enzyme concentration of 20-1000 nM is used for in vitro conjugation depending on assay conditions.
Description: Cytoplasmic region of human Hrd1 recombinant protein (Genbank Accession No. ), a.a. 236-17, with N-terminal SUMO-tag, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: PBS, 10% glycerol
Genbank: Q86TM6.2
Purity: ≥85% by SDS-PAGE
Storage Stability: Stable for≥1 year at -80°C.
Tags: N-terminal SUMO-His-tags
Uniprot: Q08109
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kaneko, M., et al. Molecular approaches to the treatment, prophylaxis, and diagnosis of Alzheimer's disease: possible involvement of HRD1, a novel molecule related to endoplasmic reticulum stress, in Alzheimer's disease. J. Pharmacol. Sci, 2012. 118(3):325-30.
2. Yagishita, N., et al. Synoviolin, protein folding and the maintenance of joint homeostasis, Nat. Clin. Prac. Rheumatol, 2008. 4(2):91-7.
3. Yamasaki, S., et al. The roles of synoviolin in crosstalk between endoplasmic reticulum stress-induced apoptosis and p53 pathway. Cell Cycle 6(11):1319-23.
Encompassing Amino Acids: 236-617
Applications: Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
Background: Hrd1 is a RING domain E3 ligase that is involved in the conjugation of ubiquitin to target substrates. Hrd1 has been demonstrated to function with the E2 enzymes UBE2D3 (UbcH5c) and UBE2G2 in vitro. Hrd1 is an ER-associated ligase involved in ERAD and has been linked to rheumatoid arthritis. It is present in human rheumatoid synovial cells and found to be a causative factor for arthropathy in in vivo studies. Hrd1 is also found in brain neurons and its proper regulation may be linked to neurodegenerative diseases. This construct is a Hrd1 N-terminal deletion containing residues 246-617 consisting of the complete C-terminal cytoplasmic portion. The N-terminal transmembrane spanning portion has been deleted. This Hrd1 N-terminal deletion mutant is active as an E3 ligase containing the RING and substrate recognition domains.
Biological Activity: Typical enzyme concentration of 20-1000 nM is used for in vitro conjugation depending on assay conditions.
Description: Cytoplasmic region of human Hrd1 recombinant protein (Genbank Accession No. ), a.a. 236-17, with N-terminal SUMO-tag, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: PBS, 10% glycerol
Genbank: Q86TM6.2
Purity: ≥85% by SDS-PAGE
Storage Stability: Stable for≥1 year at -80°C.
Tags: N-terminal SUMO-His-tags
Uniprot: Q08109
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kaneko, M., et al. Molecular approaches to the treatment, prophylaxis, and diagnosis of Alzheimer's disease: possible involvement of HRD1, a novel molecule related to endoplasmic reticulum stress, in Alzheimer's disease. J. Pharmacol. Sci, 2012. 118(3):325-30.
2. Yagishita, N., et al. Synoviolin, protein folding and the maintenance of joint homeostasis, Nat. Clin. Prac. Rheumatol, 2008. 4(2):91-7.
3. Yamasaki, S., et al. The roles of synoviolin in crosstalk between endoplasmic reticulum stress-induced apoptosis and p53 pathway. Cell Cycle 6(11):1319-23.
| Artikelnummer | BPS81051 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 81051 |
| Green Labware | Nein |
| Verpackungseinheit | 25 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) |
|
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
