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HRV 3C Protease, His-GST-tags Recombinant
HRV 3C Protease, His-GST-tags Recombinant
Artikelnummer
BPS50319
Verpackungseinheit
1000 units
Hersteller
BPS Bioscience
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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with HRV3C Protease (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 14 mM β-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa.
Description: Human rhinovirus 3Cprotease (HRV 3C Protease) with NterminalHis-GST-tags (can be easilyremoved by either Ni-chelating orGlutathione (GSH) resin along with thecleaved tag.), Recognizes the cleavagesite of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro.Cleaves between Gln and Gly. MW = 47kDa in E. coli expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0,150 mM NaCl, 1 mM EDTA, 1 mM TCEP,50% glycerol
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST-tags
Unit Definition: 1 Unit of HRV 3C Protease cleaves >95% of 100 µg of target protein at 4°C for 16 hours.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Timmer JC, Salvesen GS. Methods Mol Biol. 2011;753:243-55.
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with HRV3C Protease (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 14 mM β-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa.
Description: Human rhinovirus 3Cprotease (HRV 3C Protease) with NterminalHis-GST-tags (can be easilyremoved by either Ni-chelating orGlutathione (GSH) resin along with thecleaved tag.), Recognizes the cleavagesite of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro.Cleaves between Gln and Gly. MW = 47kDa in E. coli expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0,150 mM NaCl, 1 mM EDTA, 1 mM TCEP,50% glycerol
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST-tags
Unit Definition: 1 Unit of HRV 3C Protease cleaves >95% of 100 µg of target protein at 4°C for 16 hours.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Timmer JC, Salvesen GS. Methods Mol Biol. 2011;753:243-55.
| Artikelnummer | BPS50319 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 50319 |
| Green Labware | Nein |
| Verpackungseinheit | 1000 units |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
