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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human induced Heat Shock Protein 70 (Q435A), also known as induced HSP70 and HSP70i, with a Gln to Ala mutation on a.a. 435 of the HSP70 protein. GenBank Accession No. NM_005345, a.a. 1-641(end) with C-terminal His-tag, MW = 70.8 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 175 mM Imidazole and 20% glycerol.
Genbank: NM_005345
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P08107
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lee K.J. et al., Exp. Mol. Med. 40 (6), 658-668 (2008).
2. Birtas-Atesoglu E. et al., Clin. Exp. Rheumatol. 26, S96-S98 (2008).