HSP90β N-Terminal Domain Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Background: Hsp90β is a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90β functions has been shown to play a role in tumorigenesis and disease progression.

Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt

Description: The HSP90β Assay Kit is designed for identification of HSP90β inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant HSP90β. The key to the HSP90β Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for HSP90β reactions. The fluorescently labeled geldanamycin is incubated with a sample containing HSP90β enzyme to produce a change in fluorescent polarization. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.

Storage Stability: Stable for at least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Supplied As: The HSP90β inhibitor screening assay kit comes in a convenient 96-well format, with purified HSP90β enzyme, fluorescently labeled geldanamycin and HSP90 assay buffer for 96 enzyme reactions.

Uniprot: P08238

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Kim, J., et al., J Biomol Screening. 2004, 9(5):375-381.
2. Howes, R., et al., Anal Biochem. 2006, 350:202-213.Application Reference:
An Impermeant Ganetespib Analog Inhibits Extracellular Hsp90-Mediated Cancer Cell Migration that Involves Lysyl Oxidase 2-like Protein (2014)

• Artikelnummer: BPS50294
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50294
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK