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Human EMAP-II Recombinant
Human EMAP-II Recombinant
Artikelnummer
BPS90123-A
Verpackungseinheit
5 µg
Hersteller
BPS Bioscience
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Amino Acid Sequence: SKPI DVSRLDLRIG CIITARKHPD ADSLYVEEVDVGEIAPRTVV SGLVNHVPLE QMQNRMVILL CNLKPAKMRG VLSQAMVMCA SSPEKIEILA PPNGSVPGDR ITFDAFPGEP DKELNPKKKI WEQIQPDLHT NDECVATYKG VPFEVKGKGVCRAQTMSNSG IK
Background: EMAP-II is activated during apoptosis by cleavage of pro-EMAP through caspase-8 and enhances the recruitment of inflammatory cells by its chemotactic properties. Besides its chemotactic effect on inflammatory cells, EMAP-II increases the adhesiveness of coronary artery smooth muscle cells (CASMC) for monocytes. EMAP-II also has anti-angiogenic properties, which are explained by its pro-apoptotic effect on endothelial cells.
Biological Activity: The ED50 was determined by apoptotic effect on MCF-7 cells using a concentration of 10-50 ng/ml.
Description: Recombinant EMAP-II is a disulfide-linked monomer protein consisting of 167 amino acid residues, and migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human EMAP-II mature chain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from 0.2 µm filtered 20 mM phosphate buffer, 130 mM NaCl solution, pH 7.5.
Genbank: Q12904
Purity: ≥95% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: Q12904
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Cardiovasc Res, Feb 2008, 77: 580 - 589.
2. J. Leukoc. Biol., May 2004, 75: 772 - 776.
Amino Acid Sequence: SKPI DVSRLDLRIG CIITARKHPD ADSLYVEEVDVGEIAPRTVV SGLVNHVPLE QMQNRMVILL CNLKPAKMRG VLSQAMVMCA SSPEKIEILA PPNGSVPGDR ITFDAFPGEP DKELNPKKKI WEQIQPDLHT NDECVATYKG VPFEVKGKGVCRAQTMSNSG IK
Background: EMAP-II is activated during apoptosis by cleavage of pro-EMAP through caspase-8 and enhances the recruitment of inflammatory cells by its chemotactic properties. Besides its chemotactic effect on inflammatory cells, EMAP-II increases the adhesiveness of coronary artery smooth muscle cells (CASMC) for monocytes. EMAP-II also has anti-angiogenic properties, which are explained by its pro-apoptotic effect on endothelial cells.
Biological Activity: The ED50 was determined by apoptotic effect on MCF-7 cells using a concentration of 10-50 ng/ml.
Description: Recombinant EMAP-II is a disulfide-linked monomer protein consisting of 167 amino acid residues, and migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human EMAP-II mature chain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from 0.2 µm filtered 20 mM phosphate buffer, 130 mM NaCl solution, pH 7.5.
Genbank: Q12904
Purity: ≥95% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: Q12904
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Cardiovasc Res, Feb 2008, 77: 580 - 589.
2. J. Leukoc. Biol., May 2004, 75: 772 - 776.
| Artikelnummer | BPS90123-A |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 90123-A |
| Green Labware | Nein |
| Verpackungseinheit | 5 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) |
|
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
