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Human Fibroblast Growth Factor 10 Recombinant
Human Fibroblast Growth Factor 10 Recombinant
Artikelnummer
BPS90133-A
Verpackungseinheit
5 µg
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Amino Acid Sequence: L GQDMVSPEAT NSSSSSFSSP SSAGRHVRSY NHLQGDVRWR KLFSFTKYFL KIEKNGKVSG TKKENCPYSI LEITSVEIGV VAVKAINSNY YLAMNKKGKL YGSKEFNNDC KLKERIEENG YNTYASFNWQ HNGRQMYVAL NGKGAPRRGQ KTRRKNTSAH FLPMVVHS
Background: FGF-10 is involved in the initial budding as well as the continuous outgrowth of vertebrate limbs, FGF-10 mRNA is expressed preferentially in neurons but not in glial cells and may have a distinct role in the brain. Human FGF-10 is mitogenic for fetal rat keratinizing epidermal cells but not for NIH 3T3 cells. Recombinant FGF-10 induces the proliferation of human urothelial cells in vitro and induces the proliferation of transitional epithelium. FGF-10 is secreted by cultured mouse pre- adipocytes, prevention of FGF-10 signaling inhibits subsequent differentiation. The ability of embryonic fibroblasts derived from FGF-10 knock-out mice to differentiate into adipocytes is also impaired.
Biological Activity: The ED50 was determined by the dose-dependent proliferation of BaF3 cells expressing FGF receptors, and was found to be <0.5ng/ml., corresponding to a specific activity of 2.0 x 10^6 Units/mg.
Description: Recombinant human FGF-10, (Fibroblast Growth Factor 10), is a disulfide-linked monomer protein consisting of 169 amino acids and migrates as an approximately 19 kDa protein under reducing and non-reducing conditions. Source: Optimized DNA sequence encoding human FGF-10 mature chain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.
Genbank: O15520
Purity: ≥96% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: O15520
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Biol Reprod, Dec 2008, 79: 1226 - 1236.
2. Am. J. Respir. Cell Mol. Biol., Sep 2008, 39: 346 - 355.
3. J. Biol. Chem., Apr 2008, 283: 9308 - 9317.
Amino Acid Sequence: L GQDMVSPEAT NSSSSSFSSP SSAGRHVRSY NHLQGDVRWR KLFSFTKYFL KIEKNGKVSG TKKENCPYSI LEITSVEIGV VAVKAINSNY YLAMNKKGKL YGSKEFNNDC KLKERIEENG YNTYASFNWQ HNGRQMYVAL NGKGAPRRGQ KTRRKNTSAH FLPMVVHS
Background: FGF-10 is involved in the initial budding as well as the continuous outgrowth of vertebrate limbs, FGF-10 mRNA is expressed preferentially in neurons but not in glial cells and may have a distinct role in the brain. Human FGF-10 is mitogenic for fetal rat keratinizing epidermal cells but not for NIH 3T3 cells. Recombinant FGF-10 induces the proliferation of human urothelial cells in vitro and induces the proliferation of transitional epithelium. FGF-10 is secreted by cultured mouse pre- adipocytes, prevention of FGF-10 signaling inhibits subsequent differentiation. The ability of embryonic fibroblasts derived from FGF-10 knock-out mice to differentiate into adipocytes is also impaired.
Biological Activity: The ED50 was determined by the dose-dependent proliferation of BaF3 cells expressing FGF receptors, and was found to be <0.5ng/ml., corresponding to a specific activity of 2.0 x 10^6 Units/mg.
Description: Recombinant human FGF-10, (Fibroblast Growth Factor 10), is a disulfide-linked monomer protein consisting of 169 amino acids and migrates as an approximately 19 kDa protein under reducing and non-reducing conditions. Source: Optimized DNA sequence encoding human FGF-10 mature chain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.
Genbank: O15520
Purity: ≥96% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: O15520
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Biol Reprod, Dec 2008, 79: 1226 - 1236.
2. Am. J. Respir. Cell Mol. Biol., Sep 2008, 39: 346 - 355.
3. J. Biol. Chem., Apr 2008, 283: 9308 - 9317.
| Artikelnummer | BPS90133-A |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 90133-A |
| Green Labware | Nein |
| Verpackungseinheit | 5 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) |
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| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
