Human FLT3 Ligand Recombinant

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Encompassing Amino Acids: 27-181

Amino Acid Sequence: TQDC SFQHSPISSD FAVKIRELSD YLLQDYPVTV ASNLQDEELC GGLWRLVLAQ RWMERLKTVA GSKMQGLLER VNTEIHFVTK CAFQPPPSCL RFVQTNISRL LQETSEQLVA LKPWITRQNF SRCLELQCQP DSSTLPPPWS PRPLEATAPT A

Background: FLT-3 ligand promotes long- term expansion and differentiation of human pro-B-cells in the presence of IL-7 or in combination of IL-7 and IL-3.The human FLT-3 ligand also stimulates the proliferation of cells expressing murine FLT-3 receptors. In combination with SCF and IL-3, FLT-3 ligand can cause expansion of cells with the marker spectrum CD34(+) CD38(-). Alone, FLT-3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU- GEMM, and the very primitive high proliferative potential colony-forming cells, HPP- CFC. flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes.

Biological Activity: The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of Human AML5 cells to be in the range of 1.0 ng/ml.

Description: Recombinant FLT-3 Ligand is a disulfide-linked monomeric protein consisting of 156 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human FLT-3 ligand mature chain was expressed in E. coli.

Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Format: lyophilized protein

Formulation: Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.

Genbank: P49771

Purity: ≥97% by SDS-PAGE and HPLC

Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Uniprot: P49771

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Cancer Res. 2009 Oct 1,69(19):7747-55.
2. Blood, Jul 2009, 114: 835 - 843.
3. J. Immunol., Jun 2009, 182: 7408 - 7414.

• Artikelnummer: BPS90137-A
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 90137-A
• Verpackungseinheit: 2 µg
• Mengeneinheit: STK