Human IgA

Application Note: Human IgA whole molecule has been tested in SDS-Page and western blot and can be utilized as a control or standard reagent in SDS, Western Blotting and ELISA experiments.

Concentration Value: 1.48 mg/mL

ELISA Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Human IgA whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  Human IgA whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgA (a chain specific).   No reaction was observed against anti-Human IgG F(c).  Some light chain cross reactivity will occur with anti-Human IgG.

Background: Immunoglobulin A is the primary responder in muscosal immunity, and comprises close to 75% of the total immunoglobulin produced. IgA can also be secreted and is protected from degradation by many proteolytic enzymes (which allows it to be secreted along the gastrointestinal tract). Immunoglobulin A only weakly activates the complement system and is not readily opsonized.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC009-0106
• Hersteller: Rockland
• Hersteller Artikelnummer: 009-0106
• Verpackungseinheit: 2 mg
• Mengeneinheit: STK
• Methode: Western Blotting, SDS-PAGE
• Isotyp: IgA
• Konjugat: Unconjugated