Human IgM (myeloma) Fc5u

Application Note: Human IgM (myeloma) Fc5µ fragment has been tested in SDS-Page and can be used in Western Blotting and ELISA experiments as a control reagent.

Concentration Value: 1.0 mg/mL

ELISA Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Human IgM (myeloma) Fc5µ fragment has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography.  Purity was assessed by SDS-PAGE and HPLC to be greater than 95%.  A single precipitin arc was observed against anti-human IgM Fc5µ and anti-human serum when assayed by immuno-electrophoresis at a concentration of 20 mg/ml.  No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.

Background: Human IgM (myeloma) Fc5µ fragment consists of only the µ (mu) chain of the Fc fragment. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. IgM is formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC009-0131
• Hersteller: Rockland
• Hersteller Artikelnummer: 009-0131
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Methode: SDS-PAGE
• Isotyp: IgM
• Konjugat: Unconjugated