Human IRE1, His-Avi-tag Recombinant

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Encompassing Amino Acids: 469-977

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Description: Human serine/threonineproteinkinase/endoribonuclease IRE1(IRE1), a.a. 469-977 with a N-terminalHis-tag, TEV sequence, and AviTag™.MW = 62 kDa, expressed inbaculovirus-infected Sf9 insect cellexpression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20%glycerol, 300 mM Imidazole

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-Avi-tag

Uniprot: O75460

Warnings: Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use a carrierprotein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Urano, F., et al., Science. 2000; 287(5453): 664-666.
2. Calfon, M., et al., Nature. 2002; 415: 92- 96.

• Artikelnummer: BPS40709
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40709
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect