ICOSL:ICOS(Biotinylated) Inhibitor Screening Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: This kit is useful for screening for inhibitors of ICOSL binding to ICOS.

Background: ICOS (Inducible T-cell Costimulator, CD278) is a costimulatory molecule of the CD28 cell surface receptor superfamily that is expressed on activated T-cells. ICOS is involved in T-cell responses upon binding with its ligand, ICOSL (also known as B7-H2, CD275), which is normally expressed on B-cells, dendritic cells and monocytes. ICOS expression confers an activated phenotype and a strong suppressive capacity to intra-tumoral regulatory T-cells. In particular, the ICOS/ICOSL pathway is required for optimal antitumor responses mediated by anti-CTLA-4 therapy.

Description: The ICOSL:ICOS[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of ICOS:ICOSL signaling. The key to this kit is the high sensitivity of detection of biotin-labeled ICOS by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, ICOSL is coated on a 96-well plate. Next, ICOS is incubated with ICOSL on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Storage Stability: Up to 6 months from date of receipt, when stored as recommended.

Supplied As: This kit comes in a convenient 96- well format, with biotin-labeled ICOS, purified ICOSL, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Kieu-Suong, L., et al. Cancer Res. 2016, 76(16): 1-13.
Fu, T., et al. Cancer Res. 2011, 71(16): 1-10.

• Artikelnummer: BPS79673
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79673
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK