IDH2(R140Q) Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Useful for screening small molecular inhibitors for drug discovery and HTS applications.

Background: Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been reported in a multitude of human cancers. While the wild type IDH2, produces α-ketoglutarate (α-KG) through the reduction of NADP+ to NADPH, the IDH2(R140Q) mutant catalyzes conversion of α-ketoglutarate to 2-hydroxy-glutarate (2-HG) by oxidizing NADPH to NADP+. Genomic studies have identified the IDH2(R140Q) mutation in various cancer types, including as glioma, chondrosarcoma, AML as well as small percentage of prostate, lung and colon cancers.

Description: The IDH2(R140Q) Assay Kit is designed to measure IDH2(R140Q) activity for screening and profiling applications by measuring NADPH consumption.

Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Supplied As: The IDH2(R140Q) Assay Kit comes in a convenient 96-well format, with enough purified recombinant IDH2(R140Q) enzyme, α-KG, NADPH, NADPH detection reagents, and assay buffer for 100 enzyme reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Wang, F. et al. Science 340:622-626 (2013).

• Artikelnummer: BPS79309
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79309
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK