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Application: This kit is useful for cytokine detection in cell culture medium.
Description: IFN-γ is a cytokine that is primarily secreted by activated T cells and natural killer (NK) cells and plays an important role in activating innate/adaptive immunity. It also has been recognized as an indicator of T cell activation suggesting IFN-γ activation may be a prerequisite for CAR-T cell activity. The Colorimetric Human IFN-γ Detection Kit is designed for detecting and quantifying human interferon-γ in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IFN-γ are incubated on the coated plate followed by detecting the captured IFN-γ with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: This kit comes in a convenient 96-well format, with capture and detection antibodies for IFN-γ, streptavidin-labeled HRP, blocking buffer, IFN-γ standard, and colorimetric HRP substrate for a 96-well plate
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Billiau, A., 1996. Interferon gamma: biology and role in pathogenesis, Adv. Immunology, 62:61-130