IGFBP7, Avi-His-Tag Recombinant

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Encompassing Amino Acids: 27-282(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: IGFBP7 protein (BPS Bioscience #101545) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated CD93 protein (BPS Bioscience #101544), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Description: Recombinant human CD93, also known as complement component C1q receptor, encompassing amino acids 24-580. The C-terminus is fused with the Fc fragment of a human IgG1 followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.

Format: Aqueous buffer solution 

Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol 

Genbank: NM_001553

Host Cell Line: HEK293

Purity: ≥90%

Storage Stability: At least 6 months at -80°C. 

Tags: C-terminal Avi-His Tag

Uniprot: Q16270

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101545
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101545
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Human