IL-17A Mouse Recombinant Protein

Application Note: IL-17A protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-17A in immunological assays.

Concentration Value: 0.1 mg/mL

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Other Performance Data: Biological Activity: Mouse IL-17A is fully biologically active when compared to standards via dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts. The ED50 for recombinant mouse IL-17A in this assay was 22 ng/ml.Endotoxin Level: Measured by LAL is <0.01ng/µg or <0.1EU/µg.

Physical State: Lyophilized

Purity and Specificity: Purity was determined to be greater than 98% by RP-HPLC and by reducing and non-reducing SDS-PAGE.

Background: Mouse Interleukin-17A (IL-17A), also known as CTLA-8, is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Mouse IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and mouse IL-17A. Recombinant mouse IL-17A produced in E.coli is a non-glycosylated disulfide-joined homodimer having a molecular mass of 30.0 kDa.

Low Endotoxin: Yes

Other: User Optimized

• Artikelnummer: ROC010-001-B32
• Hersteller: Rockland
• Hersteller Artikelnummer: 010-001-B32
• Verpackungseinheit: 25 µg
• Mengeneinheit: STK
• Methode: SDS-PAGE
• Konjugat: Unconjugated