IL-17RA(Biotin):IL-17A Inhibitor Screening Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for screening small molecules and antibodies that inhibit the binding of IL-17A to IL-17RA.

Background: IL-17A is a pro-inflammatory cytokine produced by TH17 cells that plays a keyrole in inflammation, autoimmunity, and host defense. It has been shown to stimulate theproduction of pro-inflammatory cytokines, namely IL-6 and IL-8, by synoviocytes. Due to its roleas a mediator of inflammation, the IL-17 pathway has become a popular target for treatingdiseases that have a strong inflammatory component such as psoriasis, arthritis, Crohn'sdisease, and ankylosing spondylitis.

Description: The IL-17RA[Biotin]:IL-17A Inhibitor Screening Assay Kit is designed forscreening inhibitors of IL-17A:IL-17RA interaction. The IL-17RA[Biotin]-IL-17A Binding Assay Kitcomes in a convenient 96-well format, with biotin-labeled IL-17RA, purified IL-17A, streptavidinlabeledHRP, and assay buffer for 100 binding reactions. The key to this kit is the highsensitivity of detection of biotin-labeled IL-17RA by streptavidin-HRP. Only a few simple stepson a microtiter plate are required for the assay. First, IL-17A is coated on a 96-well plate. Next,IL-17RA is incubated with IL-17A on the plate. Finally, the plate is treated with streptavidin-HRPfollowed by addition of an HRP substrate to produce chemiluminescence, which can then bemeasured using a chemiluminescence reader.

Storage Stability: One year from date of receipt when stored as directed.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Iwakura Y, et al. 2008. Immunol Rev. 226:57-79.
2. Bullens DM, et al. 2013. Clin Dev Immunol. 2013:840315.
3. Ley K, et al. 2006. Immunol Res. 34(3):229-42.

• Artikelnummer: BPS72060
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 72060
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK