IL-2 Human Recombinant Protein

Application Note: IL-2 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-2 in immunological assays.

Concentration Value: 0.1 mg/mL

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Other Performance Data: Biological Activity: Recombinant Human IL-2 is fully bioactive when compared to standard. The ED50 as measured by the dose-dependent proliferation of CTLL-2 cells is typically 1.0 ng/ml.Endotoxin Level: Measured by LAL is <0.5 EU/µg.

Physical State: Lyophilized

Purity and Specificity: Purity was determined to be greater than 90% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS- PAGE, against known standard.

Background: Interleukin-2 (IL2) is a secreted immunomodulatory cytokine that is essential in regulation and proliferation of T and B lymphocytes, and other activities crucial to regulation of the immune response. The receptor of this cytokine (the IL-2R receptor) is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. Recombinant IL-2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15,517 Daltons. Recombinant human IL-2 has a Ser substitute for Cysteine at position 126.

Low Endotoxin: Yes

Other: User Optimized

• Artikelnummer: ROC009-001-B95
• Hersteller: Rockland
• Hersteller Artikelnummer: 009-001-B95
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK
• Methode: SDS-PAGE
• Konjugat: Unconjugated