Application Note: IL-32A protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-32A in immunological assays.
Concentration Value: 0.1 mg/mL
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Other Performance Data: Biological Activity: Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50 for this assay is in the range of 0.125- 1.0ug/ml.Endotoxin Level: Measured by LAL is <0.01ng/µg or <0.1EU/µg.
Physical State: Lyophilized
Purity and Specificity: Purity was determined to be greater than 97% as determined by reducing and non-reducing SDS- PAGE.
Background: Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-a, and MIP-2 production from human & mouse macrophage cell lines. It is upregulated in activated T- & NK-cells, and IFN-g-treated epithelial cells. Human recombinant IL-32 alpha produced in E. coli is a 131 amino acid non -glycosylated polypeptide with a molecular mass of 14.9 kDa.
Low Endotoxin: Yes
Other: User Optimized