English
IL12B:IL12RB1 (Biotinylated) Inhibitor Screening Chemiluminescence Assay Kit
IL12B:IL12RB1 (Biotinylated) Inhibitor Screening Chemiluminescence Assay Kit
Artikelnummer
BPS82581
Verpackungseinheit
96 reactions
Hersteller
BPS Bioscience
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Products from BPS Bioscience require a minimum order value above 400€
Application: Screen inhibitors or antibodies that block IL12B binding to IL12RB1.
Background: IL12B (Interleukin-12 subunit beta) encodes the p40 subunit of the heterodimeric cytokine IL12, which functions alongside the p35 subunit (encoded by IL12A). The IL12Rβ1 receptor subunit interacts with IL12B and plays a crucial role in the IL12 and IL23 signaling pathways, promoting Th1 cell differentiation and IFN-γ (Interferon-γ) production. Anti-p40 neutralizing antibodies, which target the p40 subunit common to both IL12 and IL23, are primarily used as therapeutic agents for autoimmune and inflammatory diseases. These antibodies have demonstrated significant efficacy in conditions such as psoriasis, psoriatic arthritis, and Crohn's disease by reducing inflammatory responses and modulating immune activity. The continued exploration of these antibodies holds promise for expanding their use to other immune-related conditions and improving patient outcomes.
Contraindications: The DMSO concentration in the final reaction should be ≤1%.
Description: The IL12B:IL12RB1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA designed for screening and profiling molecules that block the binding between IL12B (Interleukin 12B) and IL12RB1 (Interleukin 12 receptor subunit beta 1). This kit comes in a convenient 96-well format, with enough recombinant purified IL12B (amino acids 23-328(end)), biotin-labeled IL12RB1 (amino acids 24-540), streptavidin-labeled HRP, and assay buffer for 100 binding reactions.A 96-well plate is coated with IL12B protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-IL12RB1, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of IL12B to IL12RB1.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: IL12B:P29460; IL12RB1: P42701
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
Application: Screen inhibitors or antibodies that block IL12B binding to IL12RB1.
Background: IL12B (Interleukin-12 subunit beta) encodes the p40 subunit of the heterodimeric cytokine IL12, which functions alongside the p35 subunit (encoded by IL12A). The IL12Rβ1 receptor subunit interacts with IL12B and plays a crucial role in the IL12 and IL23 signaling pathways, promoting Th1 cell differentiation and IFN-γ (Interferon-γ) production. Anti-p40 neutralizing antibodies, which target the p40 subunit common to both IL12 and IL23, are primarily used as therapeutic agents for autoimmune and inflammatory diseases. These antibodies have demonstrated significant efficacy in conditions such as psoriasis, psoriatic arthritis, and Crohn's disease by reducing inflammatory responses and modulating immune activity. The continued exploration of these antibodies holds promise for expanding their use to other immune-related conditions and improving patient outcomes.
Contraindications: The DMSO concentration in the final reaction should be ≤1%.
Description: The IL12B:IL12RB1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA designed for screening and profiling molecules that block the binding between IL12B (Interleukin 12B) and IL12RB1 (Interleukin 12 receptor subunit beta 1). This kit comes in a convenient 96-well format, with enough recombinant purified IL12B (amino acids 23-328(end)), biotin-labeled IL12RB1 (amino acids 24-540), streptavidin-labeled HRP, and assay buffer for 100 binding reactions.A 96-well plate is coated with IL12B protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-IL12RB1, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of IL12B to IL12RB1.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: IL12B:P29460; IL12RB1: P42701
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS82581 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 82581 |
| Verpackungseinheit | 96 reactions |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Verwandte Artikel
Markieren Sie die Artikel, um Sie dem Warenkorb hinzuzufügen oder
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
