ISRE Reporter Kit (JAK/STAT Signaling Pathway)

Products from BPS Bioscience require a minimum order value above 400€

Applications: Monitor IFNa-induced JAK/STAT signaling pathway activity.
Screen for activators or inhibitors of the JAK/STAT signaling pathway.
Study effects of RNAi or gene overexpression on the activity of the JAK/STAT pathway.

Background: The JAK (Janus kinase) /STAT (Signal Transducer and Activator of Transcription) pathway is activated by various cytokines and growth factors and plays a critical role in cell growth, hematopoiesis, and immune response. In mammals, there are four JAKs (JAK1, JAK2, JAK3 and TYK2) and seven STAT proteins. Interferon alpha (IFNa) is a Type I interferon. Binding of IFNa to its receptor leads to the activation of JAK1 and TYK2, which in turn phosphorylate and activate STAT1 and STAT2. The phosphorylated STAT1 and 2 form a heterodimer and bind to IRF9/p48, forming a protein complex ISGF3. This complex translocates to the nucleus and binds to the ISRE (Interferon Stimulated Response Element) in the promoter region thereby promoting transcription of interferon-inducible genes.

Concentration: Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

Description: The ISRE Reporter kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection-ready ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.

Format: assay kit

Storage Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Supplied As: Reporter: ISRE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS60613
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 60613
• Green Labware: Nein
• Verpackungseinheit: 500 rxns.
• Mengeneinheit: PAK