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JAK3, His-tag Recombinant
JAK3, His-tag Recombinant
Artikelnummer
BPS40452
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 811-1124(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 40 mM Tris (pH 7.4), 10 mM MgCl2, 0.1 mg/ml BSA, 100 µM ATP, and 500 µM Jaktide as a substrate at 30°C for 20 min. ADP-Glo reagents (Promega) are used to measure the amount of ATP transferred during the catalysis.
Description: Human Jak3 (Janus kinase 3), also known as LJAK, GenBank Accession No. NM_000215, a.a. 811-1124(end) with N-terminal His-tag, MW= 37 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_000215
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P52333
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kim S. et al., Cell. Signal. 21 (6), 892-898 (2009).
2. Mullighan C.G. et al., Proc. Natl. Acad. Sci. U.S.A. 106 (23), 9414-9418 (2009).
Encompassing Amino Acids: 811-1124(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 40 mM Tris (pH 7.4), 10 mM MgCl2, 0.1 mg/ml BSA, 100 µM ATP, and 500 µM Jaktide as a substrate at 30°C for 20 min. ADP-Glo reagents (Promega) are used to measure the amount of ATP transferred during the catalysis.
Description: Human Jak3 (Janus kinase 3), also known as LJAK, GenBank Accession No. NM_000215, a.a. 811-1124(end) with N-terminal His-tag, MW= 37 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_000215
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P52333
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kim S. et al., Cell. Signal. 21 (6), 892-898 (2009).
2. Mullighan C.G. et al., Proc. Natl. Acad. Sci. U.S.A. 106 (23), 9414-9418 (2009).
| Artikelnummer | BPS40452 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40452 |
| Green Labware | Nein |
| Verpackungseinheit | 20 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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