JARID1A (KDM5A), FLAG-tag Recombinant

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Encompassing Amino Acids: 1-1090

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JARID1A (80 - 400 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Description: Human JARID1A, also known as KDM5A and RBBP2, GenBank Accession #NM_001042603), a.a. 1-1090 with C-terminal FLAG-tag, MW= 125 kDa, expressed in Sf9 cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide

Genbank: NM_001042603

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal FLAG-tag

Uniprot: P29375

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Roesch, A., et al., (2005). Mod Pathol. 18(9):1249-57.
2. Zhou, X., et al., (2010). Cancer Res. 70(10):4214-21
3. Van Zutven, LJ., et al., (2006). Genes Chromosomes Cancer 45(5):437-46.Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

• Artikelnummer: BPS50110
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50110
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect