JHDM1D (KDM7A) Chemiluminescent Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and HTS applications.

Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt

Description: The JHDM1D Chemiluminescent Assay Kit is designed to measure JHDM1Dactivity for screening and profiling applications. JHDM1D, also known as KDM7A and KIAA1718,is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylationactivity toward dimethyl-lysine 9 and dimethyl-lysine 27 on histone H3 (H3K9me2 and H3K27me2respectively). The key to the JHDM1D Chemiluminescent Assay Kit is a highly specific antibody thatrecognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate arerequired for detection of demethylase activity. First, JHDM1D enzyme is incubated with themethylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treatedwith an HRP-labeled secondary antibody followed by the addition of the HRP substrate to producechemiluminescence that can be measured using a chemiluminescence reader.

Storage Stability: Stable for at least one year from date of receipt when stored as directed. Be sure to store each component at the proper storage temperature.

Supplied As: A convenient format, with a 96-well strip plate precoated with methylated histone H3 peptide substrate, primary antibody, secondary HRP-labeled antibody, demethylase assay buffer, and purified JHDM1D for 96 enzyme reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Horton J.R., et al. Nat. Struct. & Molec. Biol. 2010; 17(1):38-43.

• Artikelnummer: BPS50612
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50612
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK