JHDM1D (KDM7A), FLAG-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 2-488

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Reaction wasperformed with 20 mM HEPES (pH 7.4),150 mM NaCl, 500 µM -ketoglutarate, 25µM iron, 2 mM ascorbic acid, 0.01%Tween-20, 0.5 µM biotinylated peptidesubstrate, and JHDM1D added to thewells. Add antibody against methylatedH3K9. Incubate for 30 min followed byAlphascreen® detection.

Description: Human JHDM1D, alsoknown as Lysine-specific demethylase 7A(KDM7A) and KIAA1718, GenBankAccession No. NM_030647 a.a. 2-488with an N-terminal FLAG-tag, MW=56kDa, expressed in a baculovirus-infectedSf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 µg/ml FLAG peptide

Genbank: NM_030647

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-tag

Uniprot: A0JNV9

Warnings: Avoidfreeze/thaw cycles. Storing dilutedenzyme is not recommended, ifnecessary, use carrier protein (BSA 0.1- 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Yang, C., et al. Cell Res. 2010. 20: 886-898.
2. Osawa, T. et al. Proc. Nat. Acad. Sci. (USA). 2011. 108(51): 20725-20729.

• Artikelnummer: BPS50419
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50419
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect