JMJD1A (KDM3A), FLAG-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 2-1322 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD1A (20 - 100 ng) added to the wells. Add antibody against mono-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Description: Human JMJD1A (also known as KDM3A or JHDM2A), GenBank Accession No. NM_018433, a.a. 2-1322 (end), with N-terminal FLAG-tag, MW=148.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ng/µl FLAG peptide, and 10% glycerol.

Genbank: NM_018433

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-tag

Uniprot: Q9Y4C1

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Du ZM. et al., PLoS One.; 6(4) : e19137 (2011).
2. Guo X. et al., Neoplasma.; 52(2): 153-157 (2011).Application Reference(s):
A scintillation proximity assay for histone demethylases (2014)

• Artikelnummer: BPS50130
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50130
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect