JMJD2A(KDM4A) (1-350), His-tag Recombinant

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Encompassing Amino Acids: 1-350

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2A (100 - 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Description: Human JMJD2A, also known as JHDM3A and KDM4A (GenBank Accession No. NM_014663) (a.a. 1-350) with N-terminal His-tag, MW=42 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, and 20% glycerol

Genbank: NM_014663

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: O75164

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Zhang, D., et al. (2005). Mol. Cell Biol. 25(15): 6404-6414.
2. Gray SG, et al. (2005). J. Biol. Chem. 280 (31): 28507-18.Application Reference(s):
A scintillation proximity assay for histone demethylases (2014)

• Artikelnummer: BPS50123
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50123
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK