JMJD2B(KDM4B) , FLAG-tag Recombinant

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Encompassing Amino Acids: 1-500

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2B (20 - 100 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Description: Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015) (a.a. 1-500) with C-terminal FLAG-tag, MW=57 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.

Genbank: NM_015015

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal FLAG-tag

Uniprot: O94953

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Fodor, B.D., et al. (2006). Genes Dev. 20(12): 1557-1562.
2. Beyer S., et al. (2008). J. Biol. Chem. 283(52):36542-52.

• Artikelnummer: BPS50104
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50104
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Insect