JMJD2B (KDM4B), GST-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 2-500)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay done using Alphascreen® technology, using BPS JMJD2B Homogeneous Assay Kit, # 50414. JMJD2B enzyme (60 ng/µl) is incubated with biotinylated histone H3 peptide substrate in JMJ assay buffer in a 10 µl reaction (50 mM HEPES (pH 8.0), 1 mM α-ketoglutarate, 80 µM FeSO4, 2 mM ascorbic acid) for 1-2 hours at RT. AlphaLISA® anti-Mouse Acceptor beads (PerkinElmer) are added, followed by the primary antibody addition and 30 min incubation at RT. Alphascreen® Streptavidin-conjugated donor beads (PerkinElmer) are added. After 10-15 minute incubation at room temperature, Alphacounts are measured.

Description: Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), a.a. 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mm KCl, 10% glycerol, 18 mM glutathione, and 3 mM DTT.

Genbank: NM_015015

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: O94953

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Fodor, B.D., et al. (2006). Genes Dev. 20(12): 1557-1562.
2. Beyer S., et al. (2008). J. Biol. Chem. 283(52):36542-52.Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

• Artikelnummer: BPS50111
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50111
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Insect