JMJD3 (KDM6B), FLAG-tag Recombinant

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Encompassing Amino Acids: 1043-end

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD3 (100 - 500 ng) added to the wells. Add antibody against demethylated K27 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Description: Human JMJD3 (KDM6B) histone demethylase (a.a. 1043-end), Genbank Accession No. NM_001080424, with C-terminal FLAG-tag, MW=71 kDa, expressed in Sf9 cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.

Genbank: NM_001080424

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal FLAG-tag

Uniprot: O15054

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Y. Xiang, et al., Cell Research (2007) 17:850-8572.
2. S. Hong, et al. Proc. Natl. Acad. Sci. USA (2007) 104(47):18439-44.
3. Swigut T, Wysocka J. Cell (2007) 131(1):29-3.Application References:
1. A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)
2. Identification of small molecule inhibitors of Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase by a sensitive high throughput screen (2013)

• Artikelnummer: BPS50115
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50115
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect