KDM5C protein-GST fusion

Application Note: KDM5C Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. KDM5C Protein is suitable for use in Western Blot. Expect a band approximately ~118kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Concentration Value: 0.1 µg/µL

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Recombinant human KDM5C (1-671) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.

Background: KDM5C or lysine (K)-specific demethylase 5C is a member of the SMCY homolog family that encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. KDM5C is mainly expressed in brain and skeletal muscle. KDM5C is involved in the regulation of transcription and chromatin remodeling that cause X-linked mental retardation (1). KDM5C is also essential for spermatogenesis and expression of male-specific minor histocompatibility antigens (2). KDM5C Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.

Low Endotoxin: No

• Artikelnummer: ROC009-001-S37S
• Hersteller: Rockland
• Hersteller Artikelnummer: 009-001-S37S
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Konjugat: Unconjugated