KHS1 (GCKR), GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: KHS1 belongs to serine/threonine kinase family that has a STE20-like protein kinase domain which stimulates the stress-activated protein kinase (SAPK, also known as Jun kinase or JNK) pathway (1). It is a mitogen-activated protein kinase kinase kinase kinase 5, termed germinal center kinase related (GCKR). Recently, the KH domain of Escherichia coli polynucleotide phosphorylase has been reported to be necessary for autoregulation and growth at low temperature (2).

Description: Recombinant human KHS1, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_006575

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q9Y4K4

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Shi, C.S. et al: TNF-mediated activation of the stress-activated protein kinase pathway: TNF receptor-associated factor 2 recruits and activates germinal center kinase related. J. Immunol. 1999;163(6):3279-85.
2. Maura Epifania, M.O. et al: The KH and S1 domains of Escherichia coli polynucleotide phosphorylase are necessary for autoregulation and growth at low temperature. Biochim. et Biophy. Acta (BBA). 2007; 1769 (3):194-203

• Artikelnummer: BPS40115
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40115
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect