KRAS (G12D), Isoform A, His-Tag, GDP-Loaded Recombinant

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Encompassing Amino Acids: 2-186

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed according to the KRAS (G12C) Coupled Nucleotide Exchange Assay Kit (BPS Bioscience #78004) with GDP loaded KRAS (G12C) (BPS Bioscience #100640) and GDP loaded KRAS (G12D) (BPS Bioscience #101312) titrated from 200 nM to 0 nM. Enzyme was incubated with SOS1; positive results show that SOS1 mediated exchange and subsequent RAS-Raf binding is successful.

Description: Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end). In this construct the N-terminal end of the protein contains a His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease BPS Bioscience #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and loaded with GDP. Unbound GDP was removed by spin column.

Format: Aqueous buffer solution

Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT

Genbank: NM_033360

Purity: ≥90%

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution

Tags: N-terminal His-tag

Uniprot: P01116

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101312
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101312
• Green Labware: Nein
• Verpackungseinheit: 4 x 50 µg
• Mengeneinheit: PAK