KRAS (G12D), Isoform A, His-Tag, GppNHp-Loaded Recombinant

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Encompassing Amino Acids: 2-186(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed according to KRAS(G12C) Coupled Nucleotide Exchange Assay Kit (BPS Bioscience #78004) without SOS1/GTP mixture. KRAS (G12D), Isoform A, His-Tag, GDP-Loaded Recombinant (BPS Bioscience #101312) and GppNHP loaded KRAS (G12D) (BPS Bioscience #101481) were titrated at various concentrations.

Description: Recombinant human KRAS (KRAS proto-oncogene GTPase), isoform a, encompassing amino acids 2-186(end). This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, BPS Bioscience #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and loaded with GppNHp, a non-hydrolyzable GTP analog. Unbound GppNHp was removed by spin column. Ready for use in KRAS-RAF binding studies or inhibitor assays.

Format: Aqueous buffer solution 

Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT 

Genbank: NM_033360

Purity: ≥90%

Storage Stability: At least 6 months at -80°C. 

Tags: N-terminal His-tag

Uniprot: P01116

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101481
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101481
• Green Labware: Nein
• Verpackungseinheit: 4 x 60 µg
• Mengeneinheit: PAK
• Wirt: Escherichia Coli