KRAS (G12V), Isoform B, His-Tag, GppNHp-Loaded Recombinant

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Encompassing Amino Acids: 2-185

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed according to KRAS(G12C) Coupled Nucleotide Exchange Assay Kit (SOS1 mediated) (>BPS Bioscience #78004) with various amounts of KRAS (G12V), His-Tag, GppNHp-Loaded.

Description: Recombinant human RAS (GTPase), isoform b, encompassing amino acids 2-185. This construct contains an N-terminal His-tag (6xHis). The protein also contains mutation of interest G12V. The protein (BPS Bioscience #100480) was affinity purified and loaded with GppNHp, a non-hydrolyzable GTP analog. Unbound GppNHp was removed by spin column.

Format: Aqueous buffer solution

Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT

Genbank: NM_004985

Purity: ≥90%

Storage Stability: At lease 6 months at -80°C

Tags: N-terminal His-tag

Uniprot: P01116

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101359
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101359
• Green Labware: Nein
• Verpackungseinheit: 4 x 60 µg
• Mengeneinheit: PAK