KRAS, Isoform B, His-Tag, GDP-loaded Recombinant

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Encompassing Amino Acids: 2-185

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed according to KRAS (G12C) Coupled Nucleotide Exchange Assay Kit (BPS Bioscience #78004) with KRAS, Isoform B, His-Tag, GDP-loaded Recombinant (BPS Bioscience #101522) titrated from at various concentrations.

Description: Recombinant human KRAS (KRAS proto-oncogene, GTPase), isoform b, encompassing amino acids 2-185. This construct contains an N-terminal His-tag (6xHis). The recombinant protein (BPS Bioscience #11308) was affinity purified and loaded with GDP. Unbound GDP was removed by spin column.

Format: Aqueous buffer solution 

Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT 

Genbank: NM_004985

Host Cell Line: Sf9 cells

Purity: ≥90%

Storage Stability: At least 6 months at -80°C. 

Tags: N-terminal His-tag

Uniprot: P01116

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101522
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101522
• Green Labware: Nein
• Verpackungseinheit: 4 x 50 µg
• Mengeneinheit: PAK
• Wirt: Insect