KRAS, Isoform B, His-Tag Recombinant

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Encompassing Amino Acids: 2-185

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Intrinsic GTPase reaction of KRAS was conducted at room temperature for 60 min using GTPase-Glo (Promega) reagents with 1 µM GTP.

Description: Human KRAS also known as C-K-RAS, CFC2, and K-RAS2A, GenBank Accession No. NM_033360, a.a. 2-185, with N-terminal His-tag, and expressed in a Baculovirus infected Sf9 cell expression system. MW=22 kDa.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 105 mM Imidazole

Genbank: NM_033360

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -70°C.

Supplied As: Aqueous buffer solution.

Tags: N-terminal His-Tag

Uniprot: P01116

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Roock, W., et al., Annals of Oncology. 2008;19(3):508-515.2. Finocchiaro, G., et al., Journal of Clinical Oncology. 2007 June; 25(18):4021.

• Artikelnummer: BPS11308
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 11308
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Insect